Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae
BACKGROUND To achieve high-level production of non-native isoprenoid products, it requires the metabolic flux to be diverted from the production of sterols to the heterologous metabolic reactions. However, there are limited tools for restricting metabolic flux towards ergosterol synthesis. In the present study, we explored dynamic control of ERG9 expression using different ergosterol-responsive...
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The yeast strain (Saccharomyces cerevisiae) MTCC 3157 was selected for combinatorial biosynthesis of plant sesquiterpene amorpha-4,11-diene. Our main objective was to overproduce amorpha 4-11-diene, which is a key precursor molecule of artemisinin (antimalarial drug) produced naturally in plant Artemisia annua through mevalonate pathway. Farnesyl diphosphate (FPP) is a common intermediate metab...
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Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2015
ISSN: 1475-2859
DOI: 10.1186/s12934-015-0220-x